====== P450 spectra ====== The spectral characterization of P450 dates back to the publication of Omura and Sato (1964). A protocol has been published by [[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843963/|Guengerich et al.(2009)]]. **P450 spectrum from insect microsomes** {{ ::locustap450.png?600 |}} \\ P450 spectrum from //Locusta migratoria// Malphighian tubule microsomes, obtained in 1976 on a Cary 118c spectrophotometer. Note the P420 shoulder. --------- **P450 spectrum from cell lysates, baculovirus expression :** \\ \\ \\ \\ \\ {{ ::6a2baculo.png?400|}} \\ \\ \\ \\ \\ P450 spectrum of CYP6A2 expressed in insect Sf21 cells with the baculovirus vector ------------- ** P450 spectra from purified insect CYP6A1** {{ ::p450_spectra.jpg?400 |}} Spectra of purified CYP6A1 from the house fly (from Andersen et al.1994). Absolute spectra (top)(this works best with clean, purified P450s) and the usual CO-difference spectrum (this works also with microsomal preparations from insect tissues, see above).