Null mutants and CRISPR/Cas knockouts and mutagenesis

P450 phenotypes or activities as revealed by null mutants and CRISPR/Cas knockouts and mutagenesis: For null mutants the phenotype is indicated. For CRISPR/Cas (indicated as CC9), the result is lower toxicity or metabolism of the listed chemical(s). Negative results are listed when available.

CYP	species	null mutants or CRISPR/Cas9	reference
CYP2 clan
CYP15B1	Aedes aegypti	CC9: null mutant viable,impairs reproductive success	Nouzova et al., 2021
CYP15C1	Bombyx mori	mod null mutant causes precocious metamorphosis, rescued by transgenic expression of wt CYP15C1	Daimon et al., 2012
CYP18A1	Drosophila melanogaster	P-element insertion causes pupal lethality	Guittard et al., 2011
CYP303A1	Drosophila melanogaster	nomph mutants disrupt development of sensory bristles	Willingham & Keil, 2004
CYP303A1	Drosophila melanogaster	null mutants cause embryonic lethality, rescued by transgenic expression of wt	Wu et al., 2019
CYP304F1	Spodoptera litura	CC9:ß-cypermethrin, chlorpyriphos,no effect on fenvalerate,cyhalothrin, phoxim	Xu et al., 2022
CYP306A1	Drosophila melanogaster	phantom (phm)E7 mutant allele causes embryonic lethality	Niwa et al., 2004; Warren et al., 2004
CYP306A1	Drosophila melanogaster	phantom (phm)null alleles block border cell migration when the entire follicular epithelium of an egg chamber is mutant	Domanitskaya et al., 2014
CYP307A1	Drosophila melanogaster	spook (spo) mutants cause embryonic lethality, rescued by transgenic expression of Drosophila or Bombyx wt	Namiki et al., 2005, Ono et al., 2006
CYP360A8	Daphnia magna	CC9:paraquat	Religia et al., 2021
CYP384A1	Tetranychus kanzawai	lemon mutant inactivates carotenoid ketolase activity	Wybouw et al., 2019
CYP384A1	Tetranychus urticae	CC9: mutant inactivates carotenoid ketolase activity: yellow colored mites	De Rouck et al., 2024
CYP3 clan
CYP6A17	Drosophila melanogaster	deltamethrin	Duneau et al., 2018
CYP6A17	Drosophila melanogaster	permethrin	Battlay et al., 2018
CYP6B207 (wrongly called CYP6B1)and CYP6B215 (wrongly called CYP6B5k)	Papilio xuthus	CC9: G0 larvae with both genes edited: growth and development inhibited after xanthotoxin feeding	Miyashita et al., 2024
CYP6D2	Drosophila melanogaster	camptothecin, but not topotecan or irinotecan	Thomas et al., 2013
CYP6D4	Drosophila melanogaster	viable, no effect on toxicity of 8 insecticides	Hardstone et al., 2006
CYP6G1	Drosophila melanogaster	CC9: imidacloprid (but not in Canton-S)	Denecke et al., 2017
CYP6G1	Drosophila melanogaster	CC9: malathion, azinphos-methyl, diazinon	Battlay et al., 2018
CYP6G2	Drosophila melanogaster	CC9:severe disruption in larval-pupal metamorphosis, reproductive deficiencies, reduced JH titer	Jia et al., 2024
CYP6T3	Drosophila melanogaster	CC9: no effect on development,no role in ecdysteroidogenesis	Shimell & O'Connor, 2022
CYP6AE14to12	Helicoverpa armigera	CC9: cluster KO, xanthotoxin, 2-tridecanone, esfenvalerate, indoxacarb	Wang et al, 2018
CYP6BQ7	Tribolium castaneum	CC9: artemisia essential oil	Zhang et al., 2021
CYP6CS1	Nilaparvata lugens	CC9: imidacloprid, thiamethoxam, nitenpyram, dinotefuran,pymetrozine,but NOT chlorpyrifos, buprofezin, triflumezopyrim	Zhang et al., 2023
CYP6ER1	Nilaparvata lugens	CC9:imidacloprid,thiacloprid,acetamiprid,nitenpyram,clothianidin,dinotefuran,flupyradifurone,sulfoxaflor	Zhang et al., 2023
CYP9A9	Spodoptera exigua	CC9:2 copies of CYP9A9, λ-cyhalothrin	Zuo et al., 2025
CYP9A98to40	Spodoptera exigua	CC9:cluster KO with CYP9A186(F116V), abamectin, emamectin benzoate	Zuo et al. 2021
CYP9A107to40	Spodoptera exigua	CC9: cluster KO, no change in resistance level	Zuo et al. 2021
CYP9A98to107	Spodoptera exigua	CC9: cluster KO with CYP9A186(F116V), abamectin, emamectin benzoate	Zuo et al. 2021
CYP9A186(F116V)	Spodoptera exigua	CC9:abamectin, emamectin benzoate	Zuo et al. 2021
CYP9A98to40	Spodoptera exigua	CC9: 10 genes cluster KO,esfenvalerate, abamectin, indoxacarb, imperatorin	Shi et al. 2023
CYP9A60to32	Spodoptera frugiperda	CC9: 13 genes cluster KO,esfenvalerate, abamectin, xanthotoxin	Shi et al. 2023
CYP9M10	Culex quinquefasciatus	TALEN knockout / permethrin	Itokawa et al., 2016
CYP4 clan
CYP4D8	Drosophila melanogaster	spinosad, but decreased toxicity of zeta-permethrin	Tabuloc et al.,2024
CYP4D14	Drosophila melanogaster	zeta-permethrin, spinosad	Tabuloc et al.,2024
CYP4D21 (wrongly called SXE1)	Drosophila melanogaster	decreased mating success of male null mutants,lipid profile in male heads altered	Fujii et al., 2008
CYP4G1	Drosophila melanogaster	null mutants die at late pupal stage / adult eclosion	Gutierrez et al., 2007
CYP4G8	Helicoverpa armigera	CC9: blocks Me-branched CHC and lethal at pupation	Xin et al., 2022
CYP4G9, G10, G26, G87	Helicoverpa armigera	CC9:cluster KO of 4G1-type: no effect noticed	Xin et al., 2022
Mito clan
CYP301A1	Drosophila melanogaster	piggyBac insertion in CYP301A1 changing C-terminal from KMTRV* to NPRKIIIL* cause abdominal cuticle defects at adult emergence	Sztal et al., 2012
CYP302A1	Drosophila melanogaster	disembodied(dib)mutants cause embryonic lethality	Warren et al. 2002
CYP314A1	Drosophila melanogaster	shade(shd)mutants cause embryonic lethality	Petryk et al 2003
CYP314A1	Drosophila melanogaster	shade(shd)null allele blocks border cell migration when the entire follicular epithelium of an egg chamber is mutant	Domanitskaya et al., 2014
CYP314A1	Drosophila melanogaster	CC9: KO cell line has elevated dengue virus 2 replication	Li et al., 2024
CYP315A1	Drosophila melanogaster	shadow (sad) mutants cause embryonic lethality	Warren et al. 2002
CYP315A1	Bombyx mori	CC9: 14 aa deletion mutants cannot molt from 2nd to 3rd instar	Zhu et al. 2021