Arthropod P450 Enchiridion

floralillusion

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-15T12:37:25+00:00

Floral volatiles allomones Larvae of the poisonous European blister beetle Meloe proscarabaeus (Coleoptera, Tenebrionoidea, Meloidae) parasitize multiple solitary bee species. These larvae (called triangula) lure bees by emitting a bouquet of volatile compounds that closely resembles floral scent. Chemical analyses reveal a complex blend of monoterpenoids derived from (S)-linalool, a ubiquitous floral volatile. These compounds function as floral-scent mimics, eliciting attraction in bees and ac…

monoterpenoids.jpg - created

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-15T12:22:28+00:00

345-347.jpg - created

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-15T12:22:07+00:00

functions

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-15T12:21:24+00:00

Arthropod P450 functions With so many arthropod species, so many P450s and so few researchers in the field, it is no surprise that we know so little about arthropod P450 functions. Even in humans, the function of 3/57 P450s is still unknown and they remain “orphans” with no known substrate (Guengerich, 2022).

nilaparvata_lugens

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-10T11:13:39+00:00

Nilaparvata lugens Class : Insecta Infraclass : Neoptera Order : Hemiptera Suborder : Auchenorrhyncha Superfamily : Fulguroidea Family : Delphacidae Common name: brown planthopper NCBI:txid108931 Annotation from assembly GCA_014356525.1, WGS project WOVF01. This assembly has some problems. A few genes were salvaged with evidence from TSA (CYP6AY1 from GAYF02039243.1; CYP6FL4 from GAYF02039105.1; CYP4C61 from GAYF02038670.1; CYP4FT1 from IACV01097039.1; CYP439…

drosophila

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-08T09:42:20+00:00

Drosophila melanogaster Class : Insecta Order : Diptera Suborder : Brachycera Clade : Cyclorrhapha Superfamily : Ephydroidea Family : Drosophilidae (subgenus Sophophora) Common name : fruit fly NCBI:txid7227 Annotation from assembly GCA_000001215.3

structure

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-08T09:36:31+00:00

P450 structure There is currently no crystal structure of an arthropod P450, but the overall structure of P450s is quite conserved, so this page provides a general background. Overview The overall P450 fold as described by Munro et al.(2013) is shown here: A clear illustration of P450 structure as it is related to the protein sequence was provided by

references

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-08T09:10:59+00:00

Selected bibliography Adesanya, A.W., Cardenas, A., Lavine, M.D., Walsh, D.B., Lavine, L.C., Zhu, F., 2020. RNA interference of NADPH-cytochrome P450 reductase increases susceptibilities to multiple acaricides in Tetranychus urticae. Pesticide Biochemistry and Physiology 165, 104550.

effects_of_p450_knockdown_by_rnai

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-08T08:57:44+00:00

Effects of P450 knockdown by RNAi The results in the table below should be interpreted with caution in the absence of independent confirmation such as in vitro activity, transgenic expression or CRISPR/Cas9 knockout. RNAi is not equally effective in all arthropod species. The effects on pesticide toxicity also need to be carefully evaluated with attention to toxicity levels in controls, levels of target gene transcript after RNAi, and significance of changes (marginal effects ?). In some cases …

null_mutants_or_crispr_cas_knockouts

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-08T08:48:16+00:00

Null mutants and CRISPR/Cas knockouts and mutagenesis P450 phenotypes or activities as revealed by null mutants and CRISPR/Cas knockouts and mutagenesis: For null mutants the phenotype is indicated. For CRISPR/Cas (indicated as CC9), the result is lower toxicity or metabolism of the listed chemical(s). Negative results are listed when available.

transgenic_expression

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-08T08:44:24+00:00

Transgenic expression in Drosophila unless indicated otherwise O: (other). Transgenic overexpression in the same organism is indicated as (OE). Result is lower toxicity or metabolism of the listed chemical(s) unless indicated otherwise (i.e. bioactivation leading to higher toxicity).

in_vitro_activities

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-08T08:38:29+00:00

In vitro activities method key: P: purified enzyme E: E.coli expression B: baculovirus expression D: Drosophila cell transfection Y: yeast expression O: other expression system. Negative results (i.e. no metabolism detected) are listed when available. These are indicative only, because improvements in assay systems can turn a negative result (e.g. metabolism below the limit of detection) to a positive one. Positive results also need to be i…

cyp307page

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-07T09:53:54+00:00

Early steps of ecdysteroid biosynthesis, the "black box" and CYP307 Biochemistry Ecdysteroid biosynthesis is generally considered to start with a sterol (e.g. C27: cholesterol; C28:campesterol; C29: sitosterol). This sterol is converted in ecdysteroidogenic cells to its Δ-7 homolog. The

cyp_clans

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-03T14:38:33+00:00

CYP clans Clans are the highest classification level of P450 genes. In a phylogenetic analysis of 2920 arthropod P450 sequences (Dermauw et al., 2020), six clans are well supported: CYP2 (100/98/68), CYP3 (100/100/74), CYP4 (98/99.7/75), CYP16 (100/100/100), CYP20 (100/100/100) and the mitochondrial clan (100/100/91). Three more clans (see below) have a more limited distribution (and are not shown in the figure).

sequenceaccess

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-03T14:37:22+00:00

Access to arthropod P450 sequences There are three ways to access arthropod P450 sequences on this website: BLAST your sequence against the arthropod P450 database using SequenceServer. Search options/parameters can be set. p450_classification Search a P450 in the database, by CYP name, clan, species, taxonomic rank or sequence.

sequences-alpha

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-03T14:36:17+00:00

Arthropod CYPomes by species, listed alphabetically * Acerentomon sp. (partial) -Hexapoda-Protura * Aculops lycopersici -Chelicerata - Arachnida * Acyrthosiphon pisum -Insecta-Hemiptera * Adoxophyes honmai -Insecta-Lepidoptera * Aedes aegypti - Insecta-Diptera * Amblyseius andersoni - Chelicerata -Arachnida * Anopheles gambiae -Insecta-Diptera * Aphis gossypii -Insecta-Hemiptera

cypfam_by_clan

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-03T14:34:38+00:00

Distribution of arthropod CYP sequences by clan Shown are 95 species with fully annotated CYPomes (in alphabetical order). The data for this table excludes pseudogenes or gene fragments, and counts alternatively spliced transcripts as a single gene. The data are specific for the genome assembly named for each

suzukii - created

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-03T14:33:43+00:00

Drosophila suzukii Class : Insecta Order : Diptera Suborder : Brachycera Clade : Cyclorrhapha Superfamily : Ephydroidea Family : Drosophilidae (subgenus Sophophora) Common name: spotted wing Drosophila NCBI:txid

suzukii.jpg - created

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-03T14:33:26+00:00

arthropod_p450_sequences

renefeyereisen (renefeyereisen@undisclosed.example.com) — 2026-09-03T14:32:20+00:00

Arthropod P450 sequences CYPome collections: For each species, a complete CYPome is provided, obtained by manual curation from genomic and transcriptomic sources. Unless noted otherwise, the CYPomes match a precise genome assembly as stated. Both the sequences and the number of genes are specific for that assembly.