Arthropod P450 Dokuwiki

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====== CYP19 clan ======
 
The CYP19 clan is named after CYP19A1, the vertebrate steroid aromatase, a highly specialized enzyme. Although it was thought that CYP19 was vertebrate-specific, CYP19-like sequences have now been found in arthropods as well. The genomic CYP19-like sequence from //Bradysia coprophila// and //Bradysia odoriphaga// are intronless and appear to result from a horizontal gene transfer (HGT) from Collembola (Feyereisen et al., 2023). CYP19 sequences are found also in some Cnidarians and Lophotrochozoa.

Key residues of the vertebrate CYP19 enzyme are conserved in the arthropod CYP19-like sequences as shown below:{{ ::cyp19align.jpg?600 |}}

For reference, human CYP19A1:

%%>%%CYP19A1-human-NP_00009400094
MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPELWKTTRPFFMKALSGPGLVRMVTVCAESLKTHLDRLEEVTNESGYVDVLTLLRRVMLDTSNTLFLRIPLDESAIVVKIQGYFDAWQALLIKPDIFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFATELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDIQKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAKNVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEMIFTPRNSDRCLEH

The following sequences are from arthropods:

Insecta-Diptera-Sciaroidea

%%>%%Bradysia_coprophila-CYP4724A1
MLRHWVNMLVHWFVYYVRPHILGFISSREVSLADTFRCNNNIKNRTWRIPELCKYLYEKYGKLVHLHLLGQRVLIVTDFKIASSLMTNHGHCLRQRFGNQFGLQNLNMHLTGLIWNNNVQQWKQNRNVFESTLKCGTKHLEALALCHLETLEKTTLKIAEPVTMLELLRNFTLSMTMEGLFEIPMPIDAQDKYDWREEAKTTVANYFKAWEFFLLNPNSQSLAENNLHQSACRKMLLFAKEIFAESEEIGSSNFISHLSHCNDNNGIFQCIAEMLLAGTDTSSITMFYTLLFLADFPEWSNKASRIIHSESQEAIDKEINHLYYESMRLIPVGPVILRQCEHDIEDGNGVSLRRGDGIIFNISGMNRANYDQPEKFNPNRYETECFPLSFGVGKKSCVGKTFAEREMKLFFTWFLKRYIVLGHQAEMISLLETRWDVANAPVQDIKLTIFPRKLIYFIGDHGTGKSSVMDAFEAAYPRIKIIRKEQVLTRQRQEKCSIQQDHPENGDLQSAVLDAHTQILNTFKSELVLIESSILDCLIKAKGKGKTCHYNKIELLRPMKKCLAVYFPMFEENQHGKQGTNKGYFDIMERAGCKSHTLKARSVSGRYEEVMNFIRKCSS

%%>%%Bradysia_odoriphaga-CYP4724A1
MLRHWVNMLVHWFIYYICPHILGFISSGGVSLADKFRCNNNIKSRTWRIPELCKYLYEKYGKLVHLHLLGQRVLIVTDFKIASSLMTNHGHCLRERFGSQFGLQNLNMHLTGLIWNNNVQQWKQNRNVFESTLKTGTKNLEALALCHLETLEKTTLKIAEPVTMLELLRNFTLSMTMEGLFEIPMPIDAQHTYDWREEAKTTVANYFKAWEFFLLNPNSQSLAENNLHQSACRKMLLFAKKIFAESQEMGSSNFISNLSHCNDNNGIFQCIAEMLLAGTDTSSITMFYTLLFLADFPEWSNKASRIINSESQEAIDKEITHLYYESMRLIPVGPVILRQCEHDIEDGDGVSLRRGDGIIFNISGMNRANYDQPEKFNPNRYETECFPLSFGVGKKSCVGKTFAEREMKLFFTWFLKRYIVLGHQAEMIGLLETRWDVANAPVQDIKLTIFPRKLIYFIGDHGTGKSSVMDAFKAAYPKIKIIRKEQVLTNHRQEKCSIQQDHLENGDLQSEFLNAHTQILNTFKSELVLIESSILDCLINAKGKVRTCDETELLRPMKKCLTVYFPTLEETQHGKQGTNKDYFDIMERAGCLSHSLKARSVSGRYEEVIDFIKKCSS 

Insecta-Zygentoma-Lepismatidae: Assembled from genome and several overlapping transcripts

%%>%%Thermobia_domestica-CYP19F1
MPYYLLVIGLLVIAATIIMKFLKKEATSNIPGPPYLGGIGSLVVFARYLWLGIPEATAYYLNKYGDTVKVWIAGEETLITSKPTIVHHVLKVNGFRYTARFGNNTGLQHLGMYHNGIIWNNDVKLWKILRAYFQKALNATTLSEAVSVSVDASKRLLQKISELRSRTDDSSIEALNFIRRITLAVTNSLMLRVNISDDEDLVQKIVDYFKAWEYFLIRPPLLYSCSHAFIKHKESVQALQEAVRQIVSNKKKILFNQNKNMDDPNLDFAEHLLFSAERGEISEEQVHQCILEMLLAGTDTSSVTLYYLLVALSENPDIEKAILKELWETLGAKDISKNDLGKLVTMEKAIKESMRIKPVGPVIMRKALENDQISGLEIRKGMNIILHVAKMHTREDIFPNPFNFLPEEHFAKNLEQEYYPFGAGPKGCVGQFLAMVEMKAILATLLRKIAFKSKNQMLKTMKTRWDIAQQPTEPTYMFFEERNEMKI 

Insecta-Zygentoma-Lepidotrichidae:(TSA)

%%>%%Tricholepidion_gertschi-GASO02049581
RTHAGAMEMWISTSCIALLAIIPLFISWLLLRRSKKGELVDIPGPSYLWGLGPYIVFARYLWMGIPEATAFYLLKYGDLVRVWIGGEQTIITSRPAVVHHVLRINGPAYTARFGNDRGLDHLGMFENGIIWNNDIPLWRILRGFFQKDEDLVERIVEYFKAWEYFLIRPPLLYKMSSSYKKHITAIQRLKDAVTDLVARKKLKMKRTGRSPTDPDCDFAEQLIFSAQQGAIKDEHICQCILEMLLAGTDTSSVTIYYLLVALSENKLVEMAVLKELSEMLEENDITKNDVPKLVTLERAIKESMRIKPVGPVIMRKALESDKVDTISIPQDTNVILHVAQMHRREEIFPQADRFLPEDHFDGKIFTKEYYPFGAGPKGCVGQFLAMVEMKAILATLLREIHFRSAGQTLQEMRTRWDIAQQPTEPTFMFFDTRKEKYSLTTS

Hexapoda-Collembola

%%>%%Folsomia_candida-4724B1-CYP19clan
MVLFPALIILVVSLLFGYVKYFRKSRGSKYFASFLQFLFHYSPQVNPFKTSSTKRINFVDNFICNYNVRHRFWMIPDLCQHISKKYGKLAELDLFGQRIVVVTDHKISNAFMTKHTKRLRQRFGNEGGLKKLNMLHTGLIWNNDVAAWKNNRAIFESTLANGARMLGELSDSYFTSTLSPSIKVGQPNSILTLLRNYTVTMTMEGLFGLDSCKSPSWRDNVKATVGNYFKAWEFFLLTPNTEQLEEEERHKKFCHAITSLSRQILASAKTTNQSKFIEDLALVKANDENQIVQCITEMLLAGTDTSSLTMFYTSLLLTDNPQVGNQLSKLINDSSTPESNTIEQMISNLYYESMRLVSVGPVILRQAEDNISLEGPDFNLKLNKGDGIVFNIAGQNLNEDPFTCAKKFDPNRYDTGGESYPLTFGTGSKSCVGKPFAEREMKLFFSWFLKKWTLLGTTEDVMGLLKTRWDIANAPLTDLKLYIFPRIGVHFIGNCETVLDTVKEFRMQFPRLQCIKLNGFDDVELAEVWEGSKNKFVIIYGGSVQEYGKVKVPDNDLIIFFENCEGEMDRECGKDANFLKYRVEKVKYNNEGGNNVRNVVDIITTRVAV

Crustacea-Copepoda-Gymnoplea-Calanoida:(TSA)

%%>%%Rhincalanus_gigas-GIVD01040536.1
MRVLQTIMTLMMMIPRGLLMIPWVSEIMFQILGIPVASRLYLNWCGDLVKVIVGGRPTIITSRFNVARHVFRGNGFNYTSRMALDSGLDMIGMLDQGIIWNNKTKDWKAMRHNFQTALGNKTLEIVEDETSAAVKMAKDTFRGGAIRNGGRLDMLAFLRLVTLEITNRLMLGVEMDNRTEVVEAVVDYFKSWEFFLIRPRILYLTSPLAFFRNRRAVQRLNDLTGDIVRKKEQEMDGADNINDVTNNFLTNLILEKRQGKISHENVVQSVLEMLIAGTDTSSVTLFYTFVALAGNKDWEENVHSEVSEVDLMAPLPSLPVTDAVLKESMRIKPVGPVVLRRSLQGDIIDELKVEAGDNIIISLEEMHKREDLFNSPEMFNPQRFLEGEKTDQGFLPFGTGPKGCVGQFLAMREMRTVMVILIKEFKLRLVAGEELGSLKVRWDIAQQPVDQIIMELIRRDVTNQDV

Crustacea-Copepoda-Gymnoplea-Calanoida:Assembled from two overlapping transcripts: GFUD01100495.1-GFUD01078968.1

%%>%%Neocalanus_flemingeri
LSRVSEVLFQLLGIPVAAKFYLSQFGDTVVVRVAGRRTLVTSSFRVSWRVFKENGFNYTSRMAEDCGLERIGMLNQGIIWNNRSKDWKKLRQYFQAAVNSRSLDMVMKHTYDSVDLVMSIHPIFQQGGGELDLLNFLRTVTLEVTNKLMFSVSMENRADLIEAIIGYF