Arthropod P450 Dokuwiki

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====== P450 spectra ======


The spectral characterization of P450 dates back to the publication of Omura and Sato (1964). A protocol has been published by [[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843963/|Guengerich et al.(2009)]].

**P450 spectrum from insect microsomes**

{{ ::locustap450.png?600 |}}

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P450 spectrum from //Locusta migratoria// Malphighian tubule microsomes, obtained in 1976 on a Cary 118c spectrophotometer. Note the P420 shoulder.



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**P450 spectrum from cell lysates, baculovirus expression :**



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{{ ::6a2baculo.png?400|}}

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P450 spectrum of CYP6A2 expressed in insect Sf21 cells with the baculovirus vector
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**
P450 spectra from purified insect CYP6A1**

{{ ::p450_spectra.jpg?400 |}}

Spectra of purified CYP6A1 from the house fly (from Andersen et al.1994). Absolute spectra (top)(this works best with clean, purified P450s) and the usual CO-difference spectrum (this works also with microsomal preparations from insect tissues, see above).